Composite

Part:BBa_K769016

Designed by: Mayumi Nakamura   Group: iGEM12_Tokyo-NoKoGen   (2012-09-19)

Promoter(high)-RBS-lux CABFEG-DT

Promoter(high)-RBS-lux CABFEG-DT (lux operon from Photobacterium phosphoreum NCMB844)
Safety.png


This part is constructed from our cloned lux operon, BBa_K769010.
The luxCABEFG is missing a luxD gene. Our aim was to make the bioluminescent reaction time faster than BBa_K769011, which is a construct where the whole lux system, luxCDABEFG is expressed under an arabinose inducible promoter. This is an intermediate of such device for shortening the reaction time. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 505
    Illegal NheI site found at 3847
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4309
    Illegal XhoI site found at 1659
    Illegal XhoI site found at 2167
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 790
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 684
    Illegal BsaI.rc site found at 1366


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